Difference between revisions of "Part:BBa K592010"
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'''iGEM11_Uppsala-Sweden:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing amilGFP BBa_K592010 (yellow), amilCP <partinfo>BBa_K592009</partinfo> (blue), and RFP <partinfo>BBa_E1010</partinfo> (red). | '''iGEM11_Uppsala-Sweden:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing amilGFP BBa_K592010 (yellow), amilCP <partinfo>BBa_K592009</partinfo> (blue), and RFP <partinfo>BBa_E1010</partinfo> (red). | ||
Revision as of 01:27, 27 September 2012
amilGFP, yellow chromoprotein
This chromoprotein from the coral Acropora millepora, amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation. The DNA was provided by Jeffrey Miller at UCLA, and was made BioBrick-compatible after removal of two illegal internal restriction sites (EcoRI and PstI).
Usage and Biology
This part is useful as a reporter.
iGEM11_Uppsala-Sweden: Expression of chromoproteins. The images above show E coli constitutively expressing amilGFP BBa_K592010 (yellow), amilCP BBa_K592009 (blue), and RFP BBa_E1010 (red).
References
[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]