Difference between revisions of "Part:BBa C2003"

 
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<partinfo>BBa_C2003 short</partinfo>
 
<partinfo>BBa_C2003 short</partinfo>
  
  
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This is protein based on Scot Wolfe's Zif268-GCN4 homodimeric zinc finger protein.  It is a fusion of fingers 2 and 3 of Zif268 (mouse) and the leucine zipper domain of GCN4 (yeast).  Scot optimized the linker between the zinc finger domain and the leucine zipper domain to increase affinity of the homodimer for the 12bp operator.
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Latest revision as of 19:13, 5 June 2006

Homodimeric zinc finger


This is protein based on Scot Wolfe's Zif268-GCN4 homodimeric zinc finger protein. It is a fusion of fingers 2 and 3 of Zif268 (mouse) and the leucine zipper domain of GCN4 (yeast). Scot optimized the linker between the zinc finger domain and the leucine zipper domain to increase affinity of the homodimer for the 12bp operator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]