Difference between revisions of "Part:BBa K909009"

 
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__NOTOC__
 
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<partinfo>BBa_K909009 short</partinfo>
 
<partinfo>BBa_K909009 short</partinfo>
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<!-- [[Image:UVR8.png|frameless|160px|right|thumb| UVR8 as a symmetric homodimer. Upon UV-B exposure the the dimer dissociates into two monomers.
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UVR8 is responsible for UV-B detection in plants. In dark state UVR8 forms a dimer, which breaks into monomers upon exposure to UV-B light (280-315 nm). Later monomer induces transcriptional changes necessary for UV-B damage repairs and protection.   
 
UVR8 is responsible for UV-B detection in plants. In dark state UVR8 forms a dimer, which breaks into monomers upon exposure to UV-B light (280-315 nm). Later monomer induces transcriptional changes necessary for UV-B damage repairs and protection.   
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In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain), to use UVR8 as a UV-B sensing domain.  
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In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain (<partinfo>BBa_K909007</partinfo>)), to use UVR8 as a UV-B sensing domain (<partinfo>BBa_K909008</partinfo>).  
  
  

Revision as of 18:26, 26 September 2012

cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana


UVR8 is responsible for UV-B detection in plants. In dark state UVR8 forms a dimer, which breaks into monomers upon exposure to UV-B light (280-315 nm). Later monomer induces transcriptional changes necessary for UV-B damage repairs and protection. In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain (BBa_K909007)), to use UVR8 as a UV-B sensing domain (BBa_K909008).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 76
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 91