Difference between revisions of "Part:BBa K907005"

Revision as of 18:08, 26 September 2012

Dual phase protein generator(mRFP default). mRFP and GFP

<Part Description>
This part is derivative of BBa_K907003, twins with BBa_K907005

BBa_E1010(mRFP, reversed) and BBa_0040(wtGFP) are attached to both ends of BBa_K907003 by overlapping extension PCR. This part normally generates mRFP in E.coli(strain MG1655). When BBa_K907000(Bxb1 integrase) inverts the promoter orientation, it starts to generate GFP.

<Part Demonstration>

Figure 1. Experimental results of BBa_K907005. Two ep-tubes designated as BBa_K907005 contain centrifuged E.coli MG1655 cells possessing BBa_K907005. When the MG1655-BBa_K907005 is double transformed with BBa_K907000, expressed in pTrcHis2A vector which means under control of Trc promoter, color of cells changed into pink rather than red of MG1655-BBa_K907005.

<Related Parts>
BBa_K907000 - Mycobacterium Phage Bxb1 integrase
BBa_K907001 - Mycobacterium Phage Bxb1 excisionase
BBa_K907002 - Binary Signal Generator, RBS(reverse) - attB - Promoter - attP - RBS
BBa_K907003 - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
BBa_K907004 - Dual Phase Protein Generator(GFP default). mRFP and GFP

Sequence and Features