Difference between revisions of "Part:BBa K733012:Design"

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===Source===
 
===Source===
  
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We digest and ligate our [https://parts.igem.org/Part:BBa_K733011 xylR+PxylA+RBS+ydcE] and [https://parts.igem.org/Part:BBa_K733010 pTms+RBS+ydcD].
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We digest and ligate our [https://parts.igem.org/Part:BBa_K733011 ''xylR''+''PxylA''+RBS+''ydcE''] and [https://parts.igem.org/Part:BBa_K733010 ''Ptms''+RBS+''ydcD''].
  
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Backbone: pTms+RBS+ydcD in pSB1C3. Enzymes used: EcoRI and XbaI.
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Backbone: ''Ptms''+RBS+''ydcD'' in pSB1C3. Enzymes used: EcoRI and XbaI.
  
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Insert: xylR+PxylA+RBS+ydcE. Enzyme used: EcoRI and SpeI. Purified by gel purification.
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Insert: ''xylR''+''PxylA''+RBS+''ydcE''. Enzyme used: EcoRI and SpeI. Purified by gel purification.
  
 
===References===
 
===References===

Latest revision as of 16:42, 26 September 2012

xylR+PxylA+RBS+ydcE+Ptms+RBS+ydcD: Cell Growth Inhibition Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.

Source

We digest and ligate our xylR+PxylA+RBS+ydcE and Ptms+RBS+ydcD.

Backbone: Ptms+RBS+ydcD in pSB1C3. Enzymes used: EcoRI and XbaI.

Insert: xylR+PxylA+RBS+ydcE. Enzyme used: EcoRI and SpeI. Purified by gel purification.

References