Difference between revisions of "Part:BBa K823030"
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We used the promtoer P<sub>''cotYZ''</sub> for the regulation of spore crust proteins in ''B. subtilis''. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-''luxABCDE'' ([[Part:BBa_K823025|BBa_K823025]]) and measured the luminescence.
 
We used the promtoer P<sub>''cotYZ''</sub> for the regulation of spore crust proteins in ''B. subtilis''. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-''luxABCDE'' ([[Part:BBa_K823025|BBa_K823025]]) and measured the luminescence.
  
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These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' [[Part:BBa_K823033|BBa_K823033]] with each two clones.
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These are results of a plate reader assay to evaluate promoter activity. P<sub>''cotYZ''</sub> was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P<sub>''cotV''</sub> [[Part:BBa_K823033|BBa_K823033]] with each two clones.
  
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[[Image:Activity PcotYZ PcotV.png|thumb|center|400px|These are results of a plate reader assay to evaluate promoter activity. P<sub>''cotYZ''</sub> was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' [[Part:BBa_K823033|BBa_K823033]] with each two clones.]]
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[[Image:Activity PcotYZ PcotV.png|thumb|center|400px|These are results of a plate reader assay to evaluate promoter activity. P<sub>''cotYZ''</sub> was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' ([[Part:BBa_K823033|BBa_K823033]]) with each two clones.]]
  
  
 
This part was used to display fusion proteins on ''B. subtilis'' spores with [[https://parts.igem.org/Part:BBa_K823049|BBa_K823049]].
 
This part was used to display fusion proteins on ''B. subtilis'' spores with [[https://parts.igem.org/Part:BBa_K823049|BBa_K823049]].
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[[Glowing_spore.png|100px|link=Part:BBa_K823049]]
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[[Glowing_spore.png|100px|link=https://parts.igem.org/Part:BBa_K823049]]
  
  

Revision as of 15:04, 26 September 2012

PcotYZ: B. subtilis promoter regulating expression of spore crust proteins

PcotYZ: Promoter of B. subtilis that is involved in spore crust formation. Hence it is activated in late sporulation. PcotYZ regulates the crust protein cotZ and cotY.

CotVWXYZ cluster. (modified after [http://www.ncbi.nlm.nih.gov/pubmed/7519271 Zhang et al., 1994)]


We used the promtoer PcotYZ for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-luxABCDE (BBa_K823025) and measured the luminescence.

These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter PcotV BBa_K823033 with each two clones.

These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter PcotV (BBa_K823033) with each two clones.


This part was used to display fusion proteins on B. subtilis spores with [[1]]. 100px|link=https://parts.igem.org/Part:BBa_K823049


See all the details about the LMU-Munich 2012 project here: http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]