Difference between revisions of "Part:BBa K823030"

Revision as of 15:02, 26 September 2012

P_cotYZ: B. subtilis promoter regulating expression of spore crust proteins

P_cotYZ: Promoter of B. subtilis that is involved in spore crust formation. Hence it is activated in late sporulation. P_cotYZ regulates the crust protein cotZ and cotY.

CotVWXYZ cluster. (modified after [http://www.ncbi.nlm.nih.gov/pubmed/7519271 Zhang et al., 1994)]

We used the promtoer P_cotYZ for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-luxABCDE (BBa_K823025) and measured the luminescence.

These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter PcotV BBa_K823033 with each two clones.

These are results of a plate reader assay to evaluate promoter activity. P_cotYZ was cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter PcotV BBa_K823033 with each two clones.

This part was used to display fusion proteins on B. subtilis spores with [[1]]. 100px|link=Part:BBa_K823049

See all the details about the LMU-Munich 2012 project here: http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K823030 short</partinfo>
-PcotYZ: B. subtilis promoter regulates spore crust proteins and is activated in late sporulation.
+P<sub>''cotYZ''</sub>: Promoter of ''B. subtilis'' that is involved in spore crust formation. Hence it is activated in late sporulation.
-PcotYZ regulates the crust protein cotZ and cotY.
+P<sub>''cotYZ''</sub> regulates the crust protein cotZ and cotY.
-We used this promoter to control the fusion proteins for display on B. subtilis spores.
 [[Image:CotVWXYZ cluster.png|thumb|400px|center|CotVWXYZ cluster. (modified after [http://www.ncbi.nlm.nih.gov/pubmed/7519271 Zhang ''et al.'', 1994)]]]
-We used the promtoer P''cotYZ'' for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-''luxABCDE'' ([[Part:BBa_K823025|BBa_K823025]]) and measured the luminescence.
+We used the promtoer P<sub>''cotYZ''</sub> for the regulation of spore crust proteins in ''B. subtilis''. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-''luxABCDE'' ([[Part:BBa_K823025|BBa_K823025]]) and measured the luminescence.
-This part was used to display fusion proteins on ''B. subtilis'' spores.
 These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' [[Part:BBa_K823033|BBa_K823033]] with each two clones.
-[[Image:Activity PcotYZ PcotV.png|thumb|center|400px|These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' [[Part:BBa_K823033|BBa_K823033]] with each two clones.]]
+[[Image:Activity PcotYZ PcotV.png|thumb|center|400px|These are results of a plate reader assay to evaluate promoter activity. P<sub>''cotYZ''</sub> was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' [[Part:BBa_K823033|BBa_K823033]] with each two clones.]]
+This part was used to display fusion proteins on ''B. subtilis'' spores with [[https://parts.igem.org/Part:BBa_K823049|BBa_K823049]].
+[[Glowing_spore.png|100px|link=Part:BBa_K823049]]