Difference between revisions of "Part:BBa K934012"
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To characterize Plas-LuxI (BBa_K934012), we introduced Plas-LuxI (BBa_K934012) with Ptrc-LasR to ''E.coli'' as “3OC12HSL dependent 3OC6HSL producer cell”. In this ''E.coli'', constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR ([https://parts.igem.org/Part:BBa_S03119 BBa_S03119]) and Plux-GFP ([https://parts.igem.org/Part:BBa_K395100 BBa_K395100]) to ''E.coli'' as a “Lux reporter cell”. | To characterize Plas-LuxI (BBa_K934012), we introduced Plas-LuxI (BBa_K934012) with Ptrc-LasR to ''E.coli'' as “3OC12HSL dependent 3OC6HSL producer cell”. In this ''E.coli'', constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR ([https://parts.igem.org/Part:BBa_S03119 BBa_S03119]) and Plux-GFP ([https://parts.igem.org/Part:BBa_K395100 BBa_K395100]) to ''E.coli'' as a “Lux reporter cell”. | ||
| − | In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, GFP expression in “Lux reporter cell” was activated. This result shows that Plas-LuxI (BBa_K934012) | + | In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, GFP expression in “Lux reporter cell” was activated. This result shows that Plas-LuxI (BBa_K934012) is fuctioning. |
We improved a previous part Plas-LuxI ([https://parts.igem.org/Part:BBa_K266000:Experience#User_Reviews BBa_K266000]) | We improved a previous part Plas-LuxI ([https://parts.igem.org/Part:BBa_K266000:Experience#User_Reviews BBa_K266000]) | ||
Revision as of 13:38, 26 September 2012
Plas-LuxI
We constructed this part by combining BBa_K649000 and BBa_K081008. This part generates LuxI enzyme in the presence of LasR-3OC12HSL complex.
To characterize Plas-LuxI (BBa_K934012), we introduced Plas-LuxI (BBa_K934012) with Ptrc-LasR to E.coli as “3OC12HSL dependent 3OC6HSL producer cell”. In this E.coli, constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR (BBa_S03119) and Plux-GFP (BBa_K395100) to E.coli as a “Lux reporter cell”.
In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, GFP expression in “Lux reporter cell” was activated. This result shows that Plas-LuxI (BBa_K934012) is fuctioning.
We improved a previous part Plas-LuxI (BBa_K266000) and accomplished a positive feedback system with our new Plux-LasI (BBa_K934022).
For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 749
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]