Difference between revisions of "Part:BBa K934012"

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[[Image:Plas-LuxI_result.png|thumb|center|500px|]]
 
[[Image:Plas-LuxI_result.png|thumb|center|500px|]]
  
To characterize Plas-LuxI (BBa_K934012), we introduced Plas-LuxI (BBa_K934012) with Ptrc-LasR to E.coli as “3OC12HSL dependent 3OC6HSL producer cell”. In this E.coli, constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR ([https://parts.igem.org/Part:BBa_S03119 BBa_S03119]) and Plux-GFP ([https://parts.igem.org/Part:BBa_K395100 BBa_K395100]) to E.coli as a “Lux reporter cell”.
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To characterize Plas-LuxI (BBa_K934012), we introduced Plas-LuxI (BBa_K934012) with Ptrc-LasR to ''E.coli'' as “3OC12HSL dependent 3OC6HSL producer cell”. In this ''E.coli'', constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR ([https://parts.igem.org/Part:BBa_S03119 BBa_S03119]) and Plux-GFP ([https://parts.igem.org/Part:BBa_K395100 BBa_K395100]) to ''E.coli'' as a “Lux reporter cell”.
  
 
In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, GFP expression in “Lux reporter cell” was activated. This result shows that Plas-LuxI (BBa_K934012) synthesized 3OC6HSL.
 
In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, GFP expression in “Lux reporter cell” was activated. This result shows that Plas-LuxI (BBa_K934012) synthesized 3OC6HSL.

Revision as of 13:23, 26 September 2012

Plas-LuxI

We constructed this part by combining BBa_K649000 and BBa_K081008. This part generates LuxI enzyme in the presence of LasR-3OC12HSL complex.


Plas-LuxI result.png

To characterize Plas-LuxI (BBa_K934012), we introduced Plas-LuxI (BBa_K934012) with Ptrc-LasR to E.coli as “3OC12HSL dependent 3OC6HSL producer cell”. In this E.coli, constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR (BBa_S03119) and Plux-GFP (BBa_K395100) to E.coli as a “Lux reporter cell”.

In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, GFP expression in “Lux reporter cell” was activated. This result shows that Plas-LuxI (BBa_K934012) synthesized 3OC6HSL.

We improved a previous part Plas-LuxI (BBa_K266000) and accomplished a positive feedback system with our new Plux-LasI (BBa_K934022).


For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 749
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]