Difference between revisions of "Part:BBa K784046"

Revision as of 10:37, 26 September 2012

theophylline riboswitch +T7*(N4) RNAP

This part contains an engineered theophylline riboswitch (RS) (BBa K784005) and an engineered T7*(N4) RNA polymerase (K784044). The riboswitch contains a RBS and it will be exposed and starts translating the T7*(N4) RNAP only after inserting of theophylline. The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7*(N4) RNAP are adjacent to one another without any restriction site between them.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 15
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K784046 short</partinfo>
-This part contains an engineered theophylline riboswitch (RS) ([https://parts.igem.org/Part:BBa_K784005 BBa K784005]) and an engineered N4 RNA polymerase ([https://parts.igem.org/Part:BBa_K784044 K784044]). The riboswitch contains a RBS and it will be exposed and starts translating the N4 RNAP only after inserting of theophylline. The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the N4 RNAP are adjacent to one another without any restriction site between them.
+This part contains an engineered theophylline riboswitch (RS) ([https://parts.igem.org/Part:BBa_K784005 BBa K784005]) and an engineered T7*(N4) RNA polymerase ([https://parts.igem.org/Part:BBa_K784044 K784044]). The riboswitch contains a RBS and it will be exposed and starts translating the T7*(N4) RNAP only after inserting of theophylline. The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7*(N4) RNAP are adjacent to one another without any restriction site between them.
 <!-- Add more about the biology of this part here