Difference between revisions of "Part:BBa K784046:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The riboswitch had to fused to the RNAP using | + | The riboswitch had to fused to the RNAP using assembly PCR. |
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===Source=== | ===Source=== | ||
Revision as of 08:41, 26 September 2012
theophylline riboswitch +T7*(N4) RNAP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 15
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The riboswitch had to fused to the RNAP using assembly PCR.
Source
Fusion PCR of BBa K784005 and K784044