Difference between revisions of "Part:BBa K907005"
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'''<Part Description>'''
 
'''<Part Description>'''
 
<br>This part is derivative of [https://parts.igem.org/Part:BBa_K907003 BBa_K907003], twins with [https://parts.igem.org/Part:BBa_K907005 BBa_K907005]
 
<br>This part is derivative of [https://parts.igem.org/Part:BBa_K907003 BBa_K907003], twins with [https://parts.igem.org/Part:BBa_K907005 BBa_K907005]
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<br>[[Image: KAIST_BBa_K907005.JPG‎ |center|500px]]
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<br>[[Image: KAIST_BBa_K907005_2.JPG‎ |center|500px]]
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[https://parts.igem.org/Part:BBa_E1010 BBa_E1010](mRFP, reversed) and [https://parts.igem.org/Part:BBa_E0040 BBa_0040](wtGFP) are attached to both ends of [https://parts.igem.org/Part:BBa_K907003 BBa_K907003] by overlapping extension PCR. This part normally generates mRFP in ''E.coli(strain MG1655)''. When [https://parts.igem.org/Part:BBa_K907000 BBa_K907000](Bxb1 integrase) inverts the promoter orientation, it starts to generate GFP.
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'''<Part Demonstration>'''
 
<br>[[Image: PPoC,_pPoCpi.png |center|500px]]
 
<br>[[Image: PPoC,_pPoCpi.png |center|500px]]
 
'''Figure 1. BBa_K907004 in MG1655 expressing GFP or mRFP.''' Left two: Default state. Center: MG1655 wild-type positive control. Right two: Inverted state.
 
'''Figure 1. BBa_K907004 in MG1655 expressing GFP or mRFP.''' Left two: Default state. Center: MG1655 wild-type positive control. Right two: Inverted state.
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<br><br>
 
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[https://parts.igem.org/Part:BBa_E1010 BBa_E1010](mRFP, reversed) and [https://parts.igem.org/Part:BBa_E0040 BBa_0040](wtGFP) are attached to both ends of [https://parts.igem.org/Part:BBa_K907003 BBa_K907003] by overlapping extension PCR. This part normally generates mRFP in ''E.coli(strain MG1655)''. When [https://parts.igem.org/Part:BBa_K907000 BBa_K907000](Bxb1 integrase) inverts the promoter orientation, it starts to generate GFP.
 
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'''<Related Parts>'''
 
'''<Related Parts>'''

Revision as of 07:56, 26 September 2012

Dual phase protein generator(mRFP default). mRFP and GFP


<Part Description>
This part is derivative of BBa_K907003, twins with BBa_K907005


500px

500px

BBa_E1010(mRFP, reversed) and BBa_0040(wtGFP) are attached to both ends of BBa_K907003 by overlapping extension PCR. This part normally generates mRFP in E.coli(strain MG1655). When BBa_K907000(Bxb1 integrase) inverts the promoter orientation, it starts to generate GFP.

<Part Demonstration>


PPoC, pPoCpi.png

Figure 1. BBa_K907004 in MG1655 expressing GFP or mRFP. Left two: Default state. Center: MG1655 wild-type positive control. Right two: Inverted state.

<Related Parts>
BBa_K907000 - Mycobacterium Phage Bxb1 integrase
BBa_K907001 - Mycobacterium Phage Bxb1 excisionase
BBa_K907002 - Binary Signal Generator, RBS(reverse) - attB - Promoter - attP - RBS
BBa_K907003 - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
BBa_K907004 - Dual Phase Protein Generator(GFP default). mRFP and GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 750
    Illegal NheI site found at 773
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 838
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 10
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 723
    Illegal BsaI.rc site found at 806
    Illegal BsaI.rc site found at 1499