Difference between revisions of "Part:BBa K907004"
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'''<Part Description>'''
 
'''<Part Description>'''
−
<br>This part is derivative of [https://parts.igem.org/Part:BBa_K907002 BBa_K907002].
+
<br>This part is derivative of [https://parts.igem.org/Part:BBa_K907002 BBa_K907002], twins with [https://parts.igem.org/Part:BBa_K907005 BBa_K907005]
  
 
<br>[[Image: PPoC,_pPoCpi.png |center|500px]]
 
<br>[[Image: PPoC,_pPoCpi.png |center|500px]]

Revision as of 20:07, 25 September 2012

Dual phase protein generator(GFP default). mRFP and GFP


<Part Description>
This part is derivative of BBa_K907002, twins with BBa_K907005


PPoC, pPoCpi.png

Figure 1. BBa_K907004 in MG1655 expressing GFP or mRFP. Left two: Default state. Center: MG1655 wild-type positive control. Right two: Inverted state.

BBa_E1010(mRFP, reversed) and BBa_E0040(wtGFP) are attached to both ends of BBa_K907002 by overlapping extension PCR. This part normally generates GFP(Left two, figure 1) in E.coli(strain MG1655). When BBa_K907000(Bxb1 integrase) inverts the promoter orientation, it starts to generate mRFP(Right two, figure 1).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 756
    Illegal NheI site found at 779
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 838
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 10
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 723
    Illegal BsaI.rc site found at 806
    Illegal BsaI.rc site found at 1499