Difference between revisions of "Part:BBa K929004"

Revision as of 09:14, 25 September 2012

modified AID+eGFP

modified_AID+eGFP

BioBrick Nr.	BBa_K929004
RFC standard	RFC 10 and aditional AgeI site
Requirement	pSB1C3
Source	existing parts: BBa_K404316 and K929001
Submitted by	[http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]

This is a composite part from the existing parts: modified_AID (BBa_K929001) and eGFP (BBa_K404316). Modified_AID (BBa_929001) is in RFC 10 cloning standard and has an aditional AgeI restriciton site. Therefore it can be fused with RFC 25 parts (C-terminal of modified AID), see Part_Design. This part was not used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see BBa_929002.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1296
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 108
Illegal BsaI.rc site found at 1225
Illegal SapI site found at 209

@@ Line 7: / Line 7: @@
 |-
 |'''BioBrick Nr.'''
-|[https://parts.igem.org/Part:BBa_929004 BBa_929004]
+|[https://parts.igem.org/Part:BBa_K929004 BBa_K929004]
 |-
 |'''RFC standard'''