Difference between revisions of "Part:BBa K729008"

Revision as of 17:59, 22 September 2012

pCstA+RBS+T7RNAP+pT7+RBS+GFP+TT

This starvation sensitive device was used by the [http://2012.igem.org/Team:University_College_London UCL’s 2012 iGem team] to increase the expression of a reporter gene (GFP) under low glucose concentrations. During carbon starvation cAMP binds the cAMP receptor protein, the complex formed activates the expression of the downstream gene (T7 RNA polymerase), which promotes the expression of GFP under the control of the T7 promoter.

This device comprises the ligation of the parts (BBa_K729007) and (BBa_I746909).

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2793
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2887

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K729008 short</partinfo>
-This starvation sensitive device was used by the ([http://2012.igem.org/Team:University_College_London UCL’s  2012]) iGem  team to increase the expression of a reporter gene (GFP) under low glucose concentrations. During carbon starvation cAMP binds the cAMP receptor protein, the complex formed activates the expression of the downstream gene (T7 RNA polymerase), which promotes the expression of GFP under the control of the T7 promoter.
+This starvation sensitive device was used by the [http://2012.igem.org/Team:University_College_London UCL’s  2012 iGem team] to increase the expression of a reporter gene (GFP) under low glucose concentrations. During carbon starvation cAMP binds the cAMP receptor protein, the complex formed activates the expression of the downstream gene (T7 RNA polymerase), which promotes the expression of GFP under the control of the T7 promoter.
 This device comprises the ligation of the parts ([https://parts.igem.org/Part:BBa_K729007 BBa_K729007]) and ([https://parts.igem.org/Part:BBa_I746909 BBa_I746909]).