Difference between revisions of "Part:BBa K929000"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K929000 short</partinfo>
 
<partinfo>BBa_K929000 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K929000 CMV_wtAID_pA ]
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|-
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! colspan="2"|[[Image:UP12_PlasmidCMV_wtAID_pA.png|300px]]
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|-
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|'''BioBrick Nr.'''
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|[https://parts.igem.org/Part:BBa_929000 BBa_929000]
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10]
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|-
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|'''Requirement'''
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|pSB1C3<br>
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|-
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|'''Source'''
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|existing parts:
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|-
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|'''Submitted by'''
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|[http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]
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|}
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<br>
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[[Image:UP12_PlasmidCMV_wtAID_pA2.png|left|thumb|400px]]<br><br><br><br><br><br><br><br><br><br><br><br><br>
  
 
The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K103001). It is built of 3 parts: CMV promoter (BBa_I712004), AID and hGH polyadenylation signal sequence (BBa_K404108).<br>
 
The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K103001). It is built of 3 parts: CMV promoter (BBa_I712004), AID and hGH polyadenylation signal sequence (BBa_K404108).<br>

Revision as of 15:13, 19 September 2012

AID with CMV promoter and hGH-polyadenylation signal sequence

CMV_wtAID_pA
UP12 PlasmidCMV wtAID pA.png
BioBrick Nr. BBa_929000
RFC standard RFC 10
Requirement pSB1C3
Source existing parts:
Submitted by [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]


UP12 PlasmidCMV wtAID pA2.png













The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K103001). It is built of 3 parts: CMV promoter (BBa_I712004), AID and hGH polyadenylation signal sequence (BBa_K404108).
AID:
AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes.
CMV promoter:
CMV stands for cytomegalovirus. The CMV promoter is commonly used due to its very strong activity, and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter.
hGH polyadenylation signal sequence:
Polyadenylation is a significant part for the translation and stability of mRNA. In eukaryotes, it is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression. hGH terminator gives a signal to start polyadenylation in the translation process.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1661
    Illegal BsaI.rc site found at 740
    Illegal SapI site found at 841