Difference between revisions of "Part:BBa K731711"

Revision as of 11:38, 19 September 2012

BBa_K731710 backbone with T7terminator (BBa_K731721)

This is the BBa_K731710 platform with the BBa_K731721 T7 terminator inserted in the BioBrick cloning region. This part was used for the characterization of both BBa_K731721 and BBa_K731710.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 6865
Illegal suffix found in sequence at 1
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 6865
Illegal NheI site found at 6894
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 6871
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 6865
Illegal BglII site found at 6011
Illegal BamHI site found at 6847
Illegal XhoI site found at 753
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 6865
Illegal suffix found in sequence at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 6865
Illegal XbaI site found at 6880
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 714
Illegal NgoMIV site found at 1051
Illegal NgoMIV site found at 4231
Illegal NgoMIV site found at 4391
Illegal NgoMIV site found at 5979
Illegal AgeI site found at 6815
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2228
Illegal SapI.rc site found at 3310

@@ Line 4: / Line 4: @@
 This is the [[Part:BBa_K731710|BBa_K731710]] platform with the [[Part:BBa_K731721|BBa_K731721]] T7 terminator inserted in the BioBrick cloning region. This part was used for the characterization of both  [[Part:BBa_K731721|BBa_K731721]] and  [[Part:BBa_K731710|BBa_K731710]].
+[[Image:K731711image.jpg]]
 <!-- Add more about the biology of this part here