Difference between revisions of "Part:BBa K784006:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K784006 short</partinfo>
 
<partinfo>BBa_K784006 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The theophylline riboswitch had to be directly fused to the start codon of the mCherry protein. To achieve this, [http://openwetware.org/wiki/Assembly_pcr Assembly_pcr] was used.
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The theophylline riboswitch had to be directly fused to the start codon of the mCherry protein. To achieve this, [http://openwetware.org/wiki/Assembly_pcr Assembly pcr] was used.
  
  

Latest revision as of 19:18, 13 September 2012

Theophylline riboswitch 12.1 + mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The theophylline riboswitch had to be directly fused to the start codon of the mCherry protein. To achieve this, [http://openwetware.org/wiki/Assembly_pcr Assembly pcr] was used.


Source

This part is a composite part of BBa_K784005 and BBa_J06504

References