Difference between revisions of "Part:BBa K823030"
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We used this promoter to control the fusion proteins for display on B. subtilis spores.
 
We used this promoter to control the fusion proteins for display on B. subtilis spores.
  
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[[Image:CotVWXYZ cluster.png|thumb|400px|left|CotVWXYZ cluster. (modified after Zhang et al., 1994)]]
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[[Image:CotVWXYZ cluster.png|thumb|400px|center|CotVWXYZ cluster. (modified after Zhang et al., 1994)]]
  
  
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We used the promtoer P''cotYZ'' for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-''luxABCDE'' ([[Part:BBa_K823025|BBa_K823025]]) and measured the luminescence.
  
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This part was used to display fusion proteins on ''B. subtilis'' spores.
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These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' [[Part:BBa_K823033|BBa_K823033]]
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[[Image:Activity PcotYZ PcotV.png|thumb|center|400px]]
  
  

Revision as of 14:15, 7 September 2012

PcotYZ: B. subtilis promoter regulating expression of spore crust proteins

PcotYZ: B. subtilis promoter regulates spore crust proteins and is activated in late sporulation. PcotYZ regulates the crust protein cotZ and cotY. We used this promoter to control the fusion proteins for display on B. subtilis spores.

CotVWXYZ cluster. (modified after Zhang et al., 1994)


We used the promtoer PcotYZ for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-luxABCDE (BBa_K823025) and measured the luminescence.

This part was used to display fusion proteins on B. subtilis spores.

These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter PcotV BBa_K823033

Activity PcotYZ PcotV.png







see all the details about the LMU-Munich 2012 project here: http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]