Difference between revisions of "Part:BBa K734001"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K734001 short</partinfo>
 
<partinfo>BBa_K734001 short</partinfo>
  
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Contains Methylxanthine N-demethylases ''NdmA'', ''NdmB'', ''NdmC'', and associated reductase ''NdmD'' from ''Psuedomonas putida'' CBB5.  Constitutive expression is driven by J23100 promoter.   
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Contains Methylxanthine N-demethylases ''NdmA'', ''NdmB'', ''NdmC'', and associated reductase ''NdmD'' from ''Psuedomonas putida'' CBB5.  Constitutive expression is driven by <partinfo>BBa_J23100</partinfo> promoter.   
  
 
Enables demethylation of 1 and 3 methylxanthines such as Theophylline (1,3-methylxanthine).   
 
Enables demethylation of 1 and 3 methylxanthines such as Theophylline (1,3-methylxanthine).   

Latest revision as of 20:55, 14 August 2012

Theophylline demethylation pathway

Contains Methylxanthine N-demethylases NdmA, NdmB, NdmC, and associated reductase NdmD from Psuedomonas putida CBB5. Constitutive expression is driven by BBa_J23100 promoter.

Enables demethylation of 1 and 3 methylxanthines such as Theophylline (1,3-methylxanthine).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 1126
    Illegal XbaI site found at 2229
    Illegal XbaI site found at 3109
    Illegal PstI site found at 548
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1229
    Illegal NheI site found at 8
    Illegal NheI site found at 31
    Illegal NheI site found at 1730
    Illegal NheI site found at 1925
    Illegal PstI site found at 548
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1229
    Illegal BglII site found at 2687
    Illegal BglII site found at 3181
    Illegal BglII site found at 3652
    Illegal XhoI site found at 4793
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 1126
    Illegal XbaI site found at 2229
    Illegal XbaI site found at 3109
    Illegal PstI site found at 548
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 1126
    Illegal XbaI site found at 2229
    Illegal XbaI site found at 3109
    Illegal PstI site found at 548
    Illegal AgeI site found at 2224
    Illegal AgeI site found at 3320
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 984
    Illegal BsaI.rc site found at 2221