Difference between revisions of "Part:BBa K566003"
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<partinfo>BBa_K566003 short</partinfo>
 
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Cyan fluorescent protein optimized from part [https://parts.igem.org/Part:BBa_E0022 BBa_E0022] with preferential codons for improved expression in <i>E. coli</i>.  
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Cyan fluorescent protein optimized from part [https://parts.igem.org/Part:BBa_E0022 BBa_E0022] with preferential codons for improved expression in <i>E. coli</i>. Is a monomeric protein generated on the basis of GFP-like protein from jellyfish <i> Aequorea macrodactyla </i>. It possesses bright fluorescence with exitation/emission maxima at 434 and 477, respectively. It has an extinction coefficient of 26,000 M-1 cm-1.
  
 
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<partinfo>BBa_K566003 parameters</partinfo>
 
<partinfo>BBa_K566003 parameters</partinfo>
 
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==References==
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<p>Patterson G., Rich N. Day and David P.(2001 Fluorescent protein spectra. CELL SCIENCE AT A GLANCE 5:837-838</p>

Revision as of 05:24, 28 September 2011

CFP optimized for E. coli

Cyan fluorescent protein optimized from part BBa_E0022 with preferential codons for improved expression in E. coli. Is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea macrodactyla . It possesses bright fluorescence with exitation/emission maxima at 434 and 477, respectively. It has an extinction coefficient of 26,000 M-1 cm-1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Patterson G., Rich N. Day and David P.(2001 Fluorescent protein spectra. CELL SCIENCE AT A GLANCE 5:837-838