Difference between revisions of "Part:BBa K516214"

Revision as of 15:34, 24 September 2011

LuxI protein generator with Ptet and RBS B0034 (TERM-)

lux I protein generator LVA-tagged driven by pTet promoter followed by BBa_B0034 high efficiency RBS.
The device is provided without Transcriptional Terminator. It could be possible use this part into plasmid vectors (such as pSB4C5) containing a Terminator in their backbones.
This is one of four systems with different RBSs provided to modulate the synthesis of the 3OC6-HSL autoinducer molecule (the other parts are: BBa_K516210 with BBa_B0030 RBS ; BBa_K516211 with BBa_B0031 RBS ; BBa_K516212 with BBa_B0032 RBS).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 717
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 lux I protein generator LVA-tagged driven by pTet promoter followed by <partinfo>B0034</partinfo> high efficiency RBS. <br>
 The device is provided without Transcriptional Terminator.
-It could be possible use this part into plasmid vectors (such as <partinfo>pSB1AK3</partinfo>) containing a Terminator in their backbones.
+It could be possible use this part into plasmid vectors (such as <partinfo>pSB4C5</partinfo>) containing a Terminator in their backbones.
 <br>
 This is one of four systems with different RBSs provided to modulate the synthesis of the 3OC6-HSL autoinducer molecule