Difference between revisions of "Part:BBa K525002:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K525002 short</partinfo>
 
<partinfo>BBa_K525002 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
Assembled  
+
Assembled via Freiburg BioBrick assembly standard (RFC 25) from <partinfo>K525403</partinfo> and <partinfo>K525560</partinfo>
  
  
  
 
===Source===
 
===Source===
 +
* <partinfo>K525403</partinfo> synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
 +
 +
* Promoter: fusion promoter between T7 promoter and lac-operator
 +
** T7 promoter from T7 phage
 +
** lac-operator from ''E. coli''
 +
 +
* <partinfo>K525401</partinfo> S-layer gene ''sbpA'' synthesized, originated in ''Lysinbacillus sphaericus'' CCM 2177
 +
 +
* <partinfo>K525499</partinfo> Ferredoxin-NADP<sup>+</sup> oxidoreductase from ''E. coli'' TOP10
 +
 +
* <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> ''bisdA'' and ''bisdB'' from ''Sphingomonas bisphenolicum'' AO1, codon optimized
 +
  
−
BBa_K525403; BBa_K525499; BBa_K123000 ; BBa_K123001 ; RCU 25
 
  
 
===References===
 
===References===
 +
Badelt-Lichtblau H, Kainz B, Völlenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk N (2009) Genetic Engineering of the S-Layer Protein SbpA of ''Lysinibacillus sphaericus'' CCM 2177 for the Generation of Functionalized Nanoarrays, ''Bioconjugate Chem'' [http://pubs.acs.org/doi/abs/10.1021/bc800445r 20(5):895–903].
 +
 +
Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in ''Sphingomonas bisphenolicum'' strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full ''J Appl Microbiol'' 105(4):1158-1169].
 +
 +
Sleytr UB, Huber C, Ilk N, Pum D, Schuster B, Egelseer EM (2007) S-layers as a tool kit for nanobiotechnological applications, [http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2006.00573.x/full ''FEMS Microbiol Lett'' 267(2):131-144].

Latest revision as of 19:00, 21 September 2011

Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal BglII site found at 221
    Illegal BamHI site found at 4539
    Illegal BamHI site found at 5277
    Illegal XhoI site found at 1996
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
    Illegal AgeI site found at 5530
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 493
    Illegal BsaI.rc site found at 622
    Illegal BsaI.rc site found at 3874


Design Notes

Assembled via Freiburg BioBrick assembly standard (RFC 25) from BBa_K525403 and BBa_K525560


Source

  • BBa_K525403 synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
  • Promoter: fusion promoter between T7 promoter and lac-operator
    • T7 promoter from T7 phage
    • lac-operator from E. coli
  • BBa_K525401 S-layer gene sbpA synthesized, originated in Lysinbacillus sphaericus CCM 2177
  • BBa_K525499 Ferredoxin-NADP+ oxidoreductase from E. coli TOP10


References

Badelt-Lichtblau H, Kainz B, Völlenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk N (2009) Genetic Engineering of the S-Layer Protein SbpA of Lysinibacillus sphaericus CCM 2177 for the Generation of Functionalized Nanoarrays, Bioconjugate Chem [http://pubs.acs.org/doi/abs/10.1021/bc800445r 20(5):895–903].

Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full J Appl Microbiol 105(4):1158-1169].

Sleytr UB, Huber C, Ilk N, Pum D, Schuster B, Egelseer EM (2007) S-layers as a tool kit for nanobiotechnological applications, [http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2006.00573.x/full FEMS Microbiol Lett 267(2):131-144].