Difference between revisions of "Part:BBa K525551:Design"
 
Line 11: Line 11:
  
 
===Source===
 
===Source===
−
* <partinfo>K52499</partinfo>: ferredoxin-NADP<sup>+</sup> oxidoreductase from ''Escherichia coli'' TOP10
+
* <partinfo>K525499</partinfo>: ferredoxin-NADP<sup>+</sup> oxidoreductase from ''Escherichia coli'' TOP10
  
 
* <partinfo>BBa_K123000</partinfo>: ''bisdA'' gene from ''Sphingomonas bisphenolicum'' AO1, probably synthesized and codon optimized (see [https://parts.igem.org/Part:BBa_K123000:Experience experience]).
 
* <partinfo>BBa_K123000</partinfo>: ''bisdA'' gene from ''Sphingomonas bisphenolicum'' AO1, probably synthesized and codon optimized (see [https://parts.igem.org/Part:BBa_K123000:Experience experience]).

Latest revision as of 16:07, 21 September 2011

NADP+ Oxidoreductase and polycistronic BisdA and BisdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1426
    Illegal BamHI site found at 2164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 789
    Illegal NgoMIV site found at 1145
    Illegal AgeI site found at 751
    Illegal AgeI site found at 1107
    Illegal AgeI site found at 2417
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 685


Design Notes

Assembled (RFC 10).


Source

  • BBa_K525499: ferredoxin-NADP+ oxidoreductase from Escherichia coli TOP10
  • BBa_K123000: bisdA gene from Sphingomonas bisphenolicum AO1, probably synthesized and codon optimized (see experience).
  • BBa_K123000: bisdB gene from S. bisphenolicum AO1, probably synthesized and codon optimized (see experience).


References