Difference between revisions of "Part:BBa K608351"

Revision as of 16:02, 21 September 2011

(correct) temperature sensitive promoter

This is based on part (BBa_K098995)

Why did we make this part?: Because sequencing of (BBa_K098995) revealed the lack of nucleotides between the RBS and start codon of the temperature sensitive λ cI repressor (BBa_K098997) CDS.

Usage and Biology

This composite promoter consists of a constitutive promoter (BBa_J23114), the temperature sensitive λ cI repressor protein (BBa_K098997 which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised to 42°C, and a cI regulated promoter based on the λ pR promoter (BBa_R0051).

The temperature sensitive promoter functioning at 28-30°C

The temperature sensitive promoter functioning at 42°C

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 781
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 <br>
-'''Why did we make this part?''': Because sequencing of ([https://parts.igem.org/Part:BBa_K098995 BBa_K098995]) revealed the lack of nucleotides between the RBS and start codon of the temperature sensitive λ cI repressor CDS.
+'''Why did we make this part?''': Because sequencing of ([https://parts.igem.org/Part:BBa_K098995 BBa_K098995]) revealed the lack of nucleotides between the RBS and start codon of the temperature sensitive λ cI repressor ([https://parts.igem.org/Part:BBa_K098997 BBa_K098997]) CDS.
 ===Usage and Biology===
-This composite promoter consists of a constitutive promoter ([https://parts.igem.org/Part:BBa_J23114 BBa_J23114]), the temperature sensitive λ cI repressor protein which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30°C to 42°C, and a cI regulated promoter based on the λ pR promoter ([https://parts.igem.org/Part:BBa_R0051 BBa_R0051]).
+This composite promoter consists of a constitutive promoter ([https://parts.igem.org/Part:BBa_J23114 BBa_J23114]), the temperature sensitive λ cI repressor protein ([https://parts.igem.org/Part:BBa_K098997 BBa_K098997] ''which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised to 42°C'', and a cI regulated promoter based on the λ pR promoter ([https://parts.igem.org/Part:BBa_R0051 BBa_R0051]).
 <br>