Difference between revisions of "Part:BBa K608351"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K608351 short</partinfo> | <partinfo>BBa_K608351 short</partinfo> | ||
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| + | This composite promoter consists of a constitutive promoter ([https://parts.igem.org/Part:BBa_J23114 BBa_J23114]), the temperature sensitive λ cI repressor protein which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30°C to 42°C, and a cI regulated promoter based on the λ pR promoter ([https://parts.igem.org/Part:BBa_R0051 BBa_R0051]). | ||
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heat sensitive cI QPI with high promoter, with 6 instead of 8bp between the RBS and cI repressor ATG | heat sensitive cI QPI with high promoter, with 6 instead of 8bp between the RBS and cI repressor ATG | ||
Revision as of 15:31, 21 September 2011
(correct) temperature sensitive promoter
This composite promoter consists of a constitutive promoter (BBa_J23114), the temperature sensitive λ cI repressor protein which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30°C to 42°C, and a cI regulated promoter based on the λ pR promoter (BBa_R0051).
heat sensitive cI QPI with high promoter, with 6 instead of 8bp between the RBS and cI repressor ATG
Usage and Biology
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The temperature sensitive promoter functioning at 28-30°C |
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Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 781 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]