Difference between revisions of "Part:BBa K592015"
m |
|||
| Line 2: | Line 2: | ||
<partinfo>BBa_K592015 short</partinfo> | <partinfo>BBa_K592015 short</partinfo> | ||
| − | This color expressing cassette is a potential alternative to BBa_J04450, the current RFP cassette in all of the BioBrick backbones. Bacterial colonies exhibit strong blue color after 18 hours. Due to the much darker blue/purple color tone, even partially blue colonies are readily recognized. | + | This color expressing cassette is a potential alternative/complementary to BBa_J04450, the current RFP cassette in all of the BioBrick backbones. Bacterial colonies exhibit strong blue color after 18 hours. Due to the much darker blue/purple color tone, even partially blue colonies are readily recognized. |
| − | We found this design very useful when assembling components that contain RFP, mCherry, etc. The assembled parts are not recognizable from the background insert if all colonies becomes red after transformation. Luckily, very few genes produce substances that make the cells turn blue/purple, which makes our construct useful. | + | We found this design very useful when assembling components that contain RFP, mCherry, etc. The assembled parts are not recognizable from the background insert if all colonies becomes red after transformation. Luckily, very few genes produce substances that make the cells turn blue/purple, which makes our construct useful. However, expressing amilCP is toxic to the cell. It's been observed that some colonies lose color eventually. |
The promoter is constitutive, the RBS is strong, the double terminator is not B0015 but B0014. | The promoter is constitutive, the RBS is strong, the double terminator is not B0015 but B0014. | ||
| Line 12: | Line 12: | ||
[[Image:Pellets BYR.jpg|300px]] | [[Image:Pellets BYR.jpg|300px]] | ||
| − | The images above show ''E coli'' constitutively expressing amilCP <partinfo>BBa_K592009</partinfo> (blue), amilGFP BBa_K592010 (yellow), and RFP <partinfo>BBa_E1010</partinfo> (red). | + | The images above show ''E coli'' constitutively expressing amilCP <partinfo>BBa_K592009</partinfo> (blue), amilGFP <partinfo>BBa_K592010</partinfo> (yellow), and RFP <partinfo>BBa_E1010</partinfo> (red). |
Revision as of 09:25, 21 September 2011
amilCP Blue Coding Device
This color expressing cassette is a potential alternative/complementary to BBa_J04450, the current RFP cassette in all of the BioBrick backbones. Bacterial colonies exhibit strong blue color after 18 hours. Due to the much darker blue/purple color tone, even partially blue colonies are readily recognized.
We found this design very useful when assembling components that contain RFP, mCherry, etc. The assembled parts are not recognizable from the background insert if all colonies becomes red after transformation. Luckily, very few genes produce substances that make the cells turn blue/purple, which makes our construct useful. However, expressing amilCP is toxic to the cell. It's been observed that some colonies lose color eventually.
The promoter is constitutive, the RBS is strong, the double terminator is not B0015 but B0014.
The images above show E coli constitutively expressing amilCP BBa_K592009 (blue), amilGFP BBa_K592010 (yellow), and RFP BBa_E1010 (red).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]