Difference between revisions of "Part:BBa K510036:Design"

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===Design Notes===
 
===Design Notes===
  
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As for the different sequences used in our construction, here it is a brief description of each one:
  
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- '''cI promoter''' ([https://parts.igem.org/Part:BBa_R0051 Part: BBa_R0051]): The cI regulated promoter is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor [https://parts.igem.org/wiki/index.php/Part:BBa_C0051 BBa_C0051]. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
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- '''RybB asRNA''': Taken from Bouvier et al. (2008). The device includes a RBS followed by the RybB asRNA and a terminator.
  
  

Revision as of 23:04, 20 September 2011

Improved Flip Flop (Module II: asRNA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As for the different sequences used in our construction, here it is a brief description of each one:

- cI promoter (Part: BBa_R0051): The cI regulated promoter is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).

- RybB asRNA: Taken from Bouvier et al. (2008). The device includes a RBS followed by the RybB asRNA and a terminator.


Source

It's a composite part which comes from different genes forming the bassic flip-flop with anothers improvements. According to Design Notes, the main source was the Registry of Standard Biological Parts although also nbci database was used to the following parts: LacI and SspB. Besides Tag sequences (DAS4, asRNA and OmpN fusion) were obtained from each articles (see references).

References

- Timothy S. G., Charles R. C. & James J. C. (2000). Construction of a genetic toggle switch in Escherichia coli. Nature 403, 339-342.

- McGinness, K.E., Baker, T.A., and Sauer, R.T. (2006). Engineering Controllable Protein Degradation. Molecular Cell 22, 701-707.

- Bouvier, M., Sharma, C.M., Mika, F., Nierhaus, K.H., and Vogel, J. (2008). Small RNA Binding to 5’ mRNA Coding Region Inhibits Translational Initiation. Molecular Cell 32, 827-837.


For further information please vistit our [http://2011.igem.org/Team:UPO-Sevilla wiki] or contact with us at igem@upo.es