Difference between revisions of "Part:BBa K510020"

Revision as of 21:53, 19 September 2011

pUC18R6KT-miniTn7BB-Gm-RFP

The mini-Tn7-Gm-RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a RFP cassette (BBa_I13521) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.

700px

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 1
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 4528
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4534
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 4528
Illegal BglII site found at 3212
Illegal BglII site found at 3483
Illegal BglII site found at 3769
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 4528
Illegal XbaI site found at 4543
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 5192
Illegal AgeI site found at 5304
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1854
Illegal SapI site found at 518

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K510020 short</partinfo>
-The mini-Tn7-Gm-RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a RFP cassette (BBa_I13521) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.
+The mini-Tn7-Gm-RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm ([https://parts.igem.org/Part:BBa_K510012 BBa_K510012]) by inserting a RFP cassette ([https://parts.igem.org/Part:BBa_I13521 BBa_I13521]) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.
+[[Image:Mini-Tn7-Gm-I13521|700px|center]]
 <!-- Add more about the biology of this part here