Difference between revisions of "Part:BBa K118025:Experience"

(GC-MS analysis of Limonene Synthase function)
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===GC-MS analysis of Limonene Synthase function===
 
===GC-MS analysis of Limonene Synthase function===
 
The limonene generator with lac promoter (<partinfo>BBa_K118025</partinfo>) includes the limonene generator (<partinfo>BBa_K118024</partinfo>) under the control of a lac promoter (<partinfo>BBa_J33207</partinfo>). This composite part's purpose is to express the limonene synthase (<partinfo>BBa_I742110</partinfo>), which is also available with a ribosome site (<partinfo>BBa_I742111</partinfo>).
 
The limonene generator with lac promoter (<partinfo>BBa_K118025</partinfo>) includes the limonene generator (<partinfo>BBa_K118024</partinfo>) under the control of a lac promoter (<partinfo>BBa_J33207</partinfo>). This composite part's purpose is to express the limonene synthase (<partinfo>BBa_I742110</partinfo>), which is also available with a ribosome site (<partinfo>BBa_I742111</partinfo>).
 
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[[Image:
{{:IGEM:British_Columbia/2011/LIMS1 GC-MS}}
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Ubcigem2011limonenegcms.jpg]]
  
 
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Revision as of 00:37, 14 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K118025

Limonene synthase expression with pRARE2 in C41 DE3 E.coli that can be used to synthesize limonene terpenes.

User Reviews

UNIQe655731571063237-partinfo-00000000-QINU UNIQe655731571063237-partinfo-00000001-QINU

Characterization

GC-MS analysis of Limonene Synthase function

The limonene generator with lac promoter (BBa_K118025) includes the limonene generator (BBa_K118024) under the control of a lac promoter (BBa_J33207). This composite part's purpose is to express the limonene synthase (BBa_I742110), which is also available with a ribosome site (BBa_I742111). [[Image: Ubcigem2011limonenegcms.jpg]]