Difference between revisions of "Part:BBa K515102"
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<partinfo>BBa_K515102 short</partinfo>
 
<partinfo>BBa_K515102 short</partinfo>
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<b>Description</b>
 
<b>Description</b>
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<p>Transmembrane chemoreceptor PA2652 coding sequence, with included optimised insulator and RBS (BBa_K515002), expression is under the control of the constitutive promoter J23100 (BBa_J23100). Device is used as an additional chemoreceptor to <i>E. coli</i> endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H).</p>
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<p>Transmembrane chemoreceptor PA2652 coding sequence, with included optimised insulator and RBS <a href="https://parts.igem.org/Part:BBa_K515002">BBa_K515002</a>, expression is under the control of the constitutive promoter J23100 (BBa_J23100). Device is used as an additional chemoreceptor to <i>E. coli</i> endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H).</p>
  
 
<b>Compatibility</b>
 
<b>Compatibility</b>
 
<p>Device has been tested in <i>E. coli DH5α</i> strain, inserted in vector backbone pSB1C3. Note that device is only functional in motile strains of <i>E. coli</i>.</p>
 
<p>Device has been tested in <i>E. coli DH5α</i> strain, inserted in vector backbone pSB1C3. Note that device is only functional in motile strains of <i>E. coli</i>.</p>
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 13:10, 11 September 2011

J23100 promoter - PA2652 Description

Transmembrane chemoreceptor PA2652 coding sequence, with included optimised insulator and RBS BBa_K515002, expression is under the control of the constitutive promoter J23100 (BBa_J23100). Device is used as an additional chemoreceptor to E. coli endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H).

Compatibility

Device has been tested in E. coli DH5α strain, inserted in vector backbone pSB1C3. Note that device is only functional in motile strains of E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 710
    Illegal NgoMIV site found at 812
    Illegal AgeI site found at 118
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1019