Difference between revisions of "Part:BBa K538000:Design"
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===References=== | ===References=== | ||
| − | # '''Ferrer ''et al.''''' Functional consequences of single:double ring transitions in chaperonins: life in the cold, ''Mol. Microbiol. 53 (1), 167-182 (2004) | + | # '''Ferrer ''et al.''''' Functional consequences of single:double ring transitions in chaperonins: life in the cold, ''Mol. Microbiol. 53 (1), 167-182'' (2004) |
| − | # '''Ferrer ''et al.''''' Expression of a Temperature-Sensitive Esterase in a Novel Chaperone-Based ''Escherichia coli'' Strain, ''App. Env. Microbiol. 70 (8), 4499-4504 (2004) | + | # '''Ferrer ''et al.''''' Expression of a Temperature-Sensitive Esterase in a Novel Chaperone-Based ''Escherichia coli'' Strain, ''App. Env. Microbiol. 70 (8), 4499-4504'' (2004) |
| − | # '''Ferrer ''et al.''''' Chaperonins govern growth of ''Escherichia coli'' at low temperatures, ''Nat. Biotech. 21, 1266 - 1267 (2003) | + | # '''Ferrer ''et al.''''' Chaperonins govern growth of ''Escherichia coli'' at low temperatures, ''Nat. Biotech. 21, 1266 - 1267'' (2003) |
| − | # '''Strocchi, Ferrer, Timmis & Golyshin''' Low temperature-induced systems failure in ''Escherichia coli'': Insights from rescue by cold-adapted chaperones, ''Proteomics 6 (1), 193-206 (2005) | + | # '''Strocchi, Ferrer, Timmis & Golyshin''' Low temperature-induced systems failure in ''Escherichia coli'': Insights from rescue by cold-adapted chaperones, ''Proteomics 6 (1), 193-206'' (2005) |
===Patents=== | ===Patents=== | ||
| − | # '''Ferrer ''et al.''''' Transgenic organisms with lower growth temperature, ''US Patent Number 7,811,784'' [http://www.google.com/patents/about?id=cGDXAAAAEBAJ (View in Google Patents)] | + | # '''Ferrer ''et al.''''' Transgenic organisms with lower growth temperature, ''US Patent Number 7,811,784'' (2004) [http://www.google.com/patents/about?id=cGDXAAAAEBAJ (View in Google Patents)] |
Revision as of 11:28, 29 July 2011
Cpn10 (O. antarctica)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 52
Design Notes
This protein has been studied extensively by Ferrer et al.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2004.04077.x/full], including its functionality in E. coli[http://aem.asm.org/cgi/content/abstract/70/8/4499][http://www.nature.com/nbt/journal/v21/n11/full/nbt1103-1266b.html][http://onlinelibrary.wiley.com/doi/10.1002/pmic.200500031/abstract], and as such its [http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts sequence] is publicly available. Pre- and suffixes were added to this as clarified in OpenWetWare's [http://openwetware.org/wiki/Biobrick_standard BioBrick standards] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10 was ensured using the [http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html EMBOSS recoder], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.
cpn10 was used by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] to create CryoBricks; cold resistance BioBricks. It encodes cochaperonin 10, a part of the cpn10/cpn60 chaperone system of Oleispira antarctica, and thus needs to be coexpressed with cpn60 (BBa_K538001) to be functional. See also the Main Page, or the team's [http://2011.igem.org/Team:Amsterdam/Project/Description Project Description] for further information.
Source
De novo synthesis by [http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/gene-synthesis/GeneArt-Gene-Synthesis.html GeneArt]
References
- Ferrer et al. Functional consequences of single:double ring transitions in chaperonins: life in the cold, Mol. Microbiol. 53 (1), 167-182 (2004)
- Ferrer et al. Expression of a Temperature-Sensitive Esterase in a Novel Chaperone-Based Escherichia coli Strain, App. Env. Microbiol. 70 (8), 4499-4504 (2004)
- Ferrer et al. Chaperonins govern growth of Escherichia coli at low temperatures, Nat. Biotech. 21, 1266 - 1267 (2003)
- Strocchi, Ferrer, Timmis & Golyshin Low temperature-induced systems failure in Escherichia coli: Insights from rescue by cold-adapted chaperones, Proteomics 6 (1), 193-206 (2005)
Patents
- Ferrer et al. Transgenic organisms with lower growth temperature, US Patent Number 7,811,784 (2004) [http://www.google.com/patents/about?id=cGDXAAAAEBAJ (View in Google Patents)]