Difference between revisions of "Part:BBa K538000:Design"
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===Source=== | ===Source=== | ||
| − | ''De novo'' synthesis | + | ''De novo'' synthesis by GeneArt[http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/gene-synthesis/GeneArt-Gene-Synthesis.html] |
===References=== | ===References=== | ||
Revision as of 12:21, 28 July 2011
Cpn10 (O. antarctica)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 52
Design Notes
The protein's sequence was published by Ferrer et al. in 2004[http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts]. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[http://openwetware.org/wiki/Biobrick_standard] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[1] was ensured using the EMBOSS recoder[http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.
Source
De novo synthesis by GeneArt[http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/gene-synthesis/GeneArt-Gene-Synthesis.html]