Difference between revisions of "Part:BBa K325909"
Line 1: Line 1:
 +
<partinfo>BBa_K325102 short</partinfo>
 +
 
<div style="font-family: Arial; padding: 00px; width: 680px; border: 0px solid #000000;">
 
<div style="font-family: Arial; padding: 00px; width: 680px; border: 0px solid #000000;">
 
{{Template:K325909 page header}}
 
{{Template:K325909 page header}}
 
{{Template:K325909 Characterization Navbar}}
 
{{Template:K325909 Characterization Navbar}}
 
<div style="padding: 00px; width: 680px; border: 0px solid #000000;">
 
<div style="padding: 00px; width: 680px; border: 0px solid #000000;">
−
<partinfo>BBa_K325102 short</partinfo>
+
 
  
 
'''Description'''<br>
 
'''Description'''<br>

Revision as of 02:57, 28 October 2010

Luciferin Regenerating Enzyme (LRE)
P. Pyralis
(E. coli optimised)

Input: L-Arabinose
Output: Light

pBad/araC
I0500		PoPS to Light
 Cambridge-Eglowli.png

Part Main Page        Arabinose -> Light        H-NS mutants        Add Data       



Description
This page described the lux operon from Vibrio fischeri. To relieve LuxR control we placed Lux C, D, A, B, E under the pBad promoter. A more complete description can be found on the [http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 Cambridge iGEM 2010 website].


Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen), [http://www.ecoliwiki.net/colipedia/index.php/BW25113 BW25113 DELTA H-NS::kan] and H-NS mutant JM 230 H-NS -205::tn10.br> Plasmids: Device has been shown to work on pSB1C3


Pictures

Figure 1 - E.coli cells (Invitrogen TOP 10) transformed with BBa K325909 in a 96 well plate.

References
[http://www.jstor.org/stable/4449975 [1]:] J. Slock, (1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri,The American Biology Teacher, 57, 225-227.

[http://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.42.100188.001055 [2]:] E.A. Meighen (1988) Enzymes and genes from the lux operons of bioluminescent bacteria, Annual Reviews in Microbiology 42, 151-176.

[http://www.annualreviews.org/doi/pdf/10.1146/annurev.ge.28.120194.001001 [3]:] E.A. Meighen, (1994) Genetics of bacterial bioluminescence, Annual Reviews of Genetics, 28, 117-139.