Difference between revisions of "Part:BBa K325909"
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'''Description'''<br>
 
'''Description'''<br>
 
This page described the ''lux operon'' from ''Vibrio fischeri''. To relieve LuxR control we placed Lux C, D, A, B, E under the [https://parts.igem.org/Part:BBa_I0500 pBad] promoter. A more complete description can be found on the [http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 Cambridge iGEM 2010 website].  
 
This page described the ''lux operon'' from ''Vibrio fischeri''. To relieve LuxR control we placed Lux C, D, A, B, E under the [https://parts.igem.org/Part:BBa_I0500 pBad] promoter. A more complete description can be found on the [http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 Cambridge iGEM 2010 website].  

Revision as of 02:57, 28 October 2010

Input: L-Arabinose
Output: Light

pBad/araC
I0500		PoPS to Light
 Cambridge-Eglowli.png

Part Main Page        Arabinose -> Light        H-NS mutants        Add Data       


Luciferin Regenerating Enzyme (LRE)
P. Pyralis
(E. coli optimised)

Description
This page described the lux operon from Vibrio fischeri. To relieve LuxR control we placed Lux C, D, A, B, E under the pBad promoter. A more complete description can be found on the [http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 Cambridge iGEM 2010 website].


Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen), [http://www.ecoliwiki.net/colipedia/index.php/BW25113 BW25113 DELTA H-NS::kan] and H-NS mutant JM 230 H-NS -205::tn10.br> Plasmids: Device has been shown to work on pSB1C3


Pictures

Figure 1 - E.coli cells (Invitrogen TOP 10) transformed with BBa K325909 in a 96 well plate.

References
[http://www.jstor.org/stable/4449975 [1]:] J. Slock, (1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri,The American Biology Teacher, 57, 225-227.

[http://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.42.100188.001055 [2]:] E.A. Meighen (1988) Enzymes and genes from the lux operons of bioluminescent bacteria, Annual Reviews in Microbiology 42, 151-176.

[http://www.annualreviews.org/doi/pdf/10.1146/annurev.ge.28.120194.001001 [3]:] E.A. Meighen, (1994) Genetics of bacterial bioluminescence, Annual Reviews of Genetics, 28, 117-139.