Difference between revisions of "Part:BBa I716211:Experience"

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10/27/10: Part works well, arabinose inducible Barnase caused cell death in a dose dependent manner. Please see http://2010.igem.org/Team:Harvard/fences/results for more info.
 
10/27/10: Part works well, arabinose inducible Barnase caused cell death in a dose dependent manner. Please see http://2010.igem.org/Team:Harvard/fences/results for more info.
 
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<B>UT-Tokyo 2015 improved this part([[part:BBa_K1631000]]) and characterize it !</B>
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We add a start codon to this part and succeeded to control the growth rate of <i>E coli(JM109)</i>.
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<p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
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[[File:Yamanaka 002.png|600px|thumb|center|alt text]]
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</p>
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We also evaluated the growth rate by slope of next graph.
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[[File:Yamanaka 001.png|600px|thumb|center|alt text]]
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<br>Plac GFP-          0.0213(/min)
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<br>Plac GFP+          0.0205(/min)
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<br>Plac Barnase GFP-  0.0222(/min)
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<br>Plac Barnase GFP+  0.0117(/min)
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Revision as of 00:47, 19 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I716211

10/27/10: This part induces cell death when expressed. We (the 2010 Harvard iGEM team) received this part from the Anderson lab at Berkeley as part of an arabinose-inducible Barnase device that also contained a constitutive Barstar. Barstar is another protein that prevents Barnase from killing the cell. In our experience Barstar is required to even be able to clone Barnase, because Barnase is so potent.

User Reviews

UNIQb8c28c95a76fb4fa-partinfo-00000000-QINU

BBa_I716211 Pmboyle

10/27/10: Part works well, arabinose inducible Barnase caused cell death in a dose dependent manner. Please see http://2010.igem.org/Team:Harvard/fences/results for more info.

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UT-Tokyo 2015 improved this part(part:BBa_K1631000) and characterize it !

We add a start codon to this part and succeeded to control the growth rate of E coli(JM109).

We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.

alt text

We also evaluated the growth rate by slope of next graph.

alt text


Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min)


UNIQb8c28c95a76fb4fa-partinfo-00000002-QINU