Translational_Unit

Part:BBa_K1631000

Designed by: Yuto Yamanaka   Group: iGEM15_UT-Tokyo   (2015-09-12)

Translational unit of Barnase

This part is improvement of Part:BBa_I716211. We added a start codon and charactarized it. If you want to know more detail, visit our wiki.
Barnase is a RNase from Bacillus amyloliquefaciens. Its cytotoxity can be neutralized by immunity protein called Barstar (Part:BBa_K1631007).
This coding sequence only contains catalitic domain (not include extracellular translocation signal peptide of Bacillus amyloliquefaciens).


Usage and Biology

We succeeded to control growth rate of E coli.

We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. We measured OD600 of 3 samples for each condition.

alt text

We also evaluated the growth rate by slope of next graph.

alt text


Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None