Difference between revisions of "Part:BBa K300005:Experience"

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In all of the measurement parts, GFP could be successfully detected in bacteria harbouring the measurement parts in high copy plasmids. For example, GFP was detected by using an excitation filter at 485nm and an emission filter at 540nm in a Infinite F200 microplate reader (Tecan).
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In all of the measurement parts, GFP could be successfully detected in bacteria harbouring the measurement parts in high copy plasmids. In this condition, GFP was detected by using an excitation filter at 485nm and an emission filter at 540nm in a Infinite F200 microplate reader (Tecan).
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 10:03, 27 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K300005

It was used do design the following BioBrick measurement systems:

to test these parts:

respectively. All of the tested parts are synthetic fusion tags whose activity could be measured by assembling a promoter with RBS upstream and a tail domain with terminator downstream, thus yielding the measurement systems. BBa_K300005 was used as a tail domain with terminator downstream. It has been assembled to test the correct folding of the resulting fusion protein by measuring the GFP and to test the affinity tag performance with a proof of concept protein.


In all of the measurement parts, GFP could be successfully detected in bacteria harbouring the measurement parts in high copy plasmids. In this condition, GFP was detected by using an excitation filter at 485nm and an emission filter at 540nm in a Infinite F200 microplate reader (Tecan).

User Reviews

UNIQ16de035235aeab2e-partinfo-0000000D-QINU UNIQ16de035235aeab2e-partinfo-0000000E-QINU