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| − | == K415031 is less leaky than R0065 ==
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| − | |[[Image:Leakiness.png]]
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| − | |The above images are FACS scatter plots of co-transformations of either pTSMa ([[Part:BBa_K415300]]) and [[Part:BBa_K415069]] or pTSMa and [[Part:BBa_K415023]]. The x-axis represents the amount of green fluorescence, and the y-axis represents the amount of red fluorescence. The parts K415069 and K415023 differ only in a single promoter sequence: K415069 has the K415031 promoter in place of a single R0065 promoter in the K415023 sequence. In this way, the more "leaky" the promoter, the more red fluorescing cells.
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| − | As you can see in the images, fewer cells with pTSMa+K415069 are leaky: 3% of total cells with pTSMa+K415069 fluoresce red, and 15% of total cells with pTSMa+K415023 fluoresce red.
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| − | |}
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| − | <!-- Add more about the biology of this part here
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| − | ===Usage and Biology===
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| − | <!-- -->
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| − | <span class='h3bb'>Sequence and Features</span>
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| − | <partinfo>BBa_K415031 SequenceAndFeatures</partinfo>
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| − | <!-- Uncomment this to enable Functional Parameter display
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| − | ===Functional Parameters===
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| − | <partinfo>BBa_K415031 parameters</partinfo>
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| − | <!-- -->
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Revision as of 06:13, 27 October 2010
Improved PLux/cI-OR Promoter
This part is a promoter that was designed as a correction to the anomalous Part:BBa_R0065 BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.
Slightly stronger binding than Part:BBa_K415032.
