Difference between revisions of "Part:BBa K411201:Design"
 
Line 11: Line 11:
  
 
===Source===
 
===Source===
−
PCR of [[Part:BBa_E0022]] to remove the LVA tag. Primers used (our [http://2010.igem.org/Team:NYMU-Taipei/ "xFP LVA remover"]):
+
Two-part PCR of [[Part:BBa_E0022]] to remove the LVA tag. Primers used:
−
*: 5' -  
+
* The inner primers(our [http://2010.igem.org/Team:NYMU-Taipei/ "xFP LVA remover"]):
−
*: 5' -  
+
** xFP_LVA_Remover_FP: 5' - gagctgtacaagaggccttaataatactagagccaggcatca - 3'
 +
** xFP_LVA_Remover_RP: 5' - tgatgcctggctctagtattattaaggcctcttgtacagctc - 3'
 +
* The outer primers (they include the biobrick prefix and suffix:
 +
** 5' -  - 3'
 +
** 5' - - 3'
 +
 
  
  
 
===References===
 
===References===

Latest revision as of 15:41, 26 October 2010

Cyan Fluorescent Protein (CFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Originated from A. victoria GFP. Has the same coding region as Part:BBa_E0021. We wanted a version of the ECFP that did not have an LVA tag.


Source

Two-part PCR of Part:BBa_E0022 to remove the LVA tag. Primers used:

  • The inner primers(our [http://2010.igem.org/Team:NYMU-Taipei/ "xFP LVA remover"]):
    • xFP_LVA_Remover_FP: 5' - gagctgtacaagaggccttaataatactagagccaggcatca - 3'
    • xFP_LVA_Remover_RP: 5' - tgatgcctggctctagtattattaaggcctcttgtacagctc - 3'
  • The outer primers (they include the biobrick prefix and suffix:
    • 5' - - 3'
    • 5' - - 3'


References