Difference between revisions of "Part:BBa K342002:Design"

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===Design Notes===
 
===Design Notes===
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<html><p>This part has been designed thanks to the results of the publication
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<html><p>This part is a self cleavable fusion protein. It has been designed thanks to the results of the publication
 
  <a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1"><font color= black><i>Banki et al.</i><font>
 
  <a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1"><font color= black><i>Banki et al.</i><font>
 
</a>The second phasin increases the rate of purified protein during the step of granules extraction.The intein is a self cleavable protein which breaks under PH=6 and allows the release od the fused protein at the end of the sequence</p>
 
</a>The second phasin increases the rate of purified protein during the step of granules extraction.The intein is a self cleavable protein which breaks under PH=6 and allows the release od the fused protein at the end of the sequence</p>

Revision as of 14:48, 25 October 2010

Phasin-Phasin Intein (for silver standard fusion)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1693
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1279
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Unknown


Design Notes

This part is a self cleavable fusion protein. It has been designed thanks to the results of the publication Banki et al. The second phasin increases the rate of purified protein during the step of granules extraction.The intein is a self cleavable protein which breaks under PH=6 and allows the release od the fused protein at the end of the sequence

The phasin sequence is the same as the biobrick BBa_K208001 from the Utah 2009 team.

The sequencing of this part is difficult because of the repetition of phasin's sequence.

Source

Ligation of two synthetized parts : Phasin and Phasin-Intein

References