Difference between revisions of "Part:BBa K316021"
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For reliable expression of spectinomycin resistance gene (Aad9 <bbpart>BBa_K143031</bbpart>) in B. subtilis, the strongest constitutive promoter Pveg and strong ribosome binding site spoVG <bbpart>BBa_K143053</bbpart> were used. A double terminator <bbpart>BBa_B0015</bbpart> ensures there is minimal read-through. | For reliable expression of spectinomycin resistance gene (Aad9 <bbpart>BBa_K143031</bbpart>) in B. subtilis, the strongest constitutive promoter Pveg and strong ribosome binding site spoVG <bbpart>BBa_K143053</bbpart> were used. A double terminator <bbpart>BBa_B0015</bbpart> ensures there is minimal read-through. | ||
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+ | [[Image:SpecCasette.PNG|center|800px]] | ||
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+ | '''Figure I.''' Spectinomycin resistance cassette. | ||
Revision as of 18:25, 27 October 2010
Spectinomycin resistance with promoter for B.subtilis
For reliable expression of spectinomycin resistance gene (Aad9 BBa_K143031) in B. subtilis, the strongest constitutive promoter Pveg and strong ribosome binding site spoVG BBa_K143053 were used. A double terminator BBa_B0015 ensures there is minimal read-through.
Figure I. Spectinomycin resistance cassette.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 611