Difference between revisions of "Part:BBa K316021"
Line 2: | Line 2: | ||
<partinfo>BBa_K316021 short</partinfo> | <partinfo>BBa_K316021 short</partinfo> | ||
− | For reliable expression of spectinomycin resistance gene in B. subtilis, the strongest constitutive promoter Pveg and strong ribosome binding site spoVG <bbpart>BBa_K143053</bbpart> were used. A double terminator <bbpart>BBa_B0015</bbpart> ensures there is minimal read-through. | + | For reliable expression of spectinomycin resistance gene (Aad9 <bbpart>BBa_K143031</bbpart>) in B. subtilis, the strongest constitutive promoter Pveg and strong ribosome binding site spoVG <bbpart>BBa_K143053</bbpart> were used. A double terminator <bbpart>BBa_B0015</bbpart> ensures there is minimal read-through. |
Revision as of 10:17, 25 October 2010
Spectinomycin resistance with promoter for B.subtilis
For reliable expression of spectinomycin resistance gene (Aad9 BBa_K143031) in B. subtilis, the strongest constitutive promoter Pveg and strong ribosome binding site spoVG BBa_K143053 were used. A double terminator BBa_B0015 ensures there is minimal read-through.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 611