Difference between revisions of "Part:BBa K389002:Design"
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===Design Notes=== | ===Design Notes=== | ||
* point mutations G56V and I77V so it works without ''rpoA'' gene from ''Agrobacterium tumefaciens'' in ''Escherichia coli'' (YC Jung ''et. al.'', 2004) | * point mutations G56V and I77V so it works without ''rpoA'' gene from ''Agrobacterium tumefaciens'' in ''Escherichia coli'' (YC Jung ''et. al.'', 2004) | ||
| + | * RBS (<partinfo>B0034</partinfo>-like) included | ||
| + | * additional stop codon TAA added | ||
* synthesized by Mr. Gene, so: | * synthesized by Mr. Gene, so: | ||
** optimated codon usage for ''E. coli'' | ** optimated codon usage for ''E. coli'' | ||
Revision as of 14:49, 5 October 2010
Mutated virG
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- point mutations G56V and I77V so it works without rpoA gene from Agrobacterium tumefaciens in Escherichia coli (YC Jung et. al., 2004)
- RBS (BBa_B0034-like) included
- additional stop codon TAA added
- synthesized by Mr. Gene, so:
- optimated codon usage for E. coli
- removal of illegal restriction sites
Source
synthetic
References
YC Jung et al. (2004) Mutants of Agrobacterium tumefaciens virG Gene That Activate Transcription of vir Promoter in Escherichia coli, Current Microbiol 49:334-340.