Difference between revisions of "Part:pSB1C3"
Line 2: Line 2:
 
<partinfo>pSB1C3 short</partinfo>
 
<partinfo>pSB1C3 short</partinfo>
  
−
This is a high copy plasmid for use in assembly of BioBrick&reg; standard biological parts.
+
High copy BioBrick assembly plasmid
  
−
===Usage and Biology===
+
pSB1C3 is a high copy number plasmid carrying chloramphenicol resistance.
  
−
Download a genbank file of this part: [[Image:PSB1C3-P1010.gb]]
+
The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
 +
 
 +
===Usage and Biology===
  
 
<!-- -->
 
<!-- -->

Revision as of 17:49, 4 May 2011

High copy BioBrick assembly plasmid

High copy BioBrick assembly plasmid

pSB1C3 is a high copy number plasmid carrying chloramphenicol resistance.

The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2049
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2049
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2049
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2064
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.