Difference between revisions of "Part:BBa K404201"

Revision as of 15:03, 31 August 2010

ViralBrick-453-BAP

ViralBrick-453-BAP
BioBrick Nr.	BBa_K404201
RFC standard	RFC 25RFC 10
Backbone	pSB1C3_VCK
Length
Source	synthetic
Submitted by	[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]

All capsid coding parts of the Virus Construction Kit designed by the iGEM Team Freiburg contain single cutting restriction sites around the major loop sequences. Using these restriction sites it is possible to insert functional motifs into the coding sequence for the viral capsid.
This contains one of these functional motifs fanked by bilatera linkers and the viral sequences that code for the loop. This category of BioBricks was termed ViralBrick to clarify that cloning does not function with the usual iGEM RFC restriction sites but with the mentioned ViralBrick restriction sits.

Schematic representation of the BioBrick with all relevant annotations

ViralBricks as a convenient possibility to insert functional mofits into the viral capsid

Function principle of Viral Bricks

Risk assessment and terms of use

Viral Systems based on the Adeno-associated Virus of the serotype 2 are to handle under Biosafety level 1 according to the german [http://bundesrecht.juris.de/gentg/index.html Act on Genetic Engineering] and the specification of the ZKBS on working with the AAV. This general classification is restricted to experiments that use the ITRs (Inverted Terminal Repeats) of the AAV2 and do not contain hazzardous gene sequences on the vector plasmid.
Please consider special provisions of law in your contry before using parts that contribute the production of genetically modified viral vectors

Sequence and Features