Difference between revisions of "Part:BBa K404201"
VolkerMorath (Talk | contribs) |
VolkerMorath (Talk | contribs) |
||
| Line 24: | Line 24: | ||
|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] | |[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] | ||
|} | |} | ||
| + | |||
| + | All capsid coding parts of the Virus Construction Kit designed by the iGEM Team Freiburg contain single cutting restriction sites around the major loop sequences. Using these restriction sites it is possible to insert functional motifs into the coding sequence for the viral capsid.<br> | ||
| + | This part | ||
<br><br><br><br> | <br><br><br><br> | ||
| Line 33: | Line 36: | ||
====ViralBricks as a convenient possibility to insert functional mofits into the viral capsid==== | ====ViralBricks as a convenient possibility to insert functional mofits into the viral capsid==== | ||
| − | [[Image:Freiburg10_ViralBricks.png|thumb|right| | + | [[Image:Freiburg10_ViralBricks.png|thumb|right|360px| Function principle of Viral Bricks]] |
<br><br><br><br> | <br><br><br><br> | ||
Revision as of 14:47, 31 August 2010
ViralBrick-453-BAP
| ViralBrick-453-BAP | |
|---|---|
| |
| BioBrick Nr. | BBa_K404201 |
| RFC standard | RFC 25RFC 10 |
| Backbone | pSB1C3_VCK |
| Length | |
| Source | synthetic |
| Submitted by | [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] |
All capsid coding parts of the Virus Construction Kit designed by the iGEM Team Freiburg contain single cutting restriction sites around the major loop sequences. Using these restriction sites it is possible to insert functional motifs into the coding sequence for the viral capsid.
This part
ViralBricks as a convenient possibility to insert functional mofits into the viral capsid
Risk assessment and terms of use
Viral Systems based on the Adeno-associated Virus of the serotype 2 are to handle under Biosafety level 1 according to the german [http://bundesrecht.juris.de/gentg/index.html Act on Genetic Engineering] and the specification of the ZKBS on working with the AAV. This general classification is restricted to experiments that use the ITRs (Inverted Terminal Repeats) of the AAV2 and do not contain hazzardous gene sequences on the vector plasmid.
Please consider special provisions of law in your contry before using parts that contribute the production of genetically modified viral vectors
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]