Difference between revisions of "Part:BBa J70628"

 
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<partinfo>BBa_J70628 short</partinfo>
 
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This is a RFC12 compatible Intein self-splicing protein.  It is capable of self cleavage induced by overnight incubation at 4°C with 50 mM DDT.
  
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===Usage and Biology===
 
===Usage and Biology===
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Intein domains are commonly fused with CBD (<partinfo>J70627</partinfo>) and then used to cleave off the CBD tag after purification, yielding very high levels of the target protein. Note that the target protein's N terminal or C terminal residue can affect cleavage.
  
 
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Revision as of 20:42, 11 August 2010

RFC12 Sce VMA Intein Internal Domain This is a RFC12 compatible Intein self-splicing protein. It is capable of self cleavage induced by overnight incubation at 4°C with 50 mM DDT.

Usage and Biology

Intein domains are commonly fused with CBD (BBa_J70627) and then used to cleave off the CBD tag after purification, yielding very high levels of the target protein. Note that the target protein's N terminal or C terminal residue can affect cleavage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 486
    Illegal BamHI site found at 1339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1195
  • 1000
    COMPATIBLE WITH RFC[1000]