Difference between revisions of "Part:BBa J70592:Design"
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Latest revision as of 13:33, 3 August 2010
RFC12 Constitutive Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There was a NheI site present at bp 7, so a silent mutation was introduced at that location to change a T to a G.
Designed with synthetic oligos:
5' AATTC GCGGCCGC T ACTAGT TTGACGGCGAGCTCAGTCCTAGGTACAGTGCTAGC GCTAGC A GCGGCCG CTGCA 3', Prom-F 5' GCGGCCGCTGCTAGC GCTAGCACTGTACCTAGGACTGAGCTCGCCGTCAA ACTAGTAGCGGCCGCG 3', Prom-R
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.