Difference between revisions of "Part:BBa J70611:Design"
(→Design Notes) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J70611 short</partinfo> | <partinfo>BBa_J70611 short</partinfo> | ||
| Line 9: | Line 8: | ||
PCR amplified off of <partinfo>BBa_J04450</partinfo> with primers: | PCR amplified off of <partinfo>BBa_J04450</partinfo> with primers: | ||
| − | GTTTCTT C GAATTC GCGGCCGC T ACTAGT GCTTCCTCCGAAGACGTTATCAAAGAG, RFP-F | + | GTTTCTT C GAATTC GCGGCCGC T ACTAGT GCTTCCTCCGAAGACGTTATCAAAGAG, RFP-F |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| + | GTTTCTT C CTGCAG CGGCCGC T GCTAGC AGCACCGGTGGAGTGACGAC, RFP-R | ||
===Source=== | ===Source=== | ||
Latest revision as of 20:30, 20 July 2010
RFC12 RFP Internal Domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 552
Illegal AgeI site found at 664 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR amplified off of BBa_J04450 with primers:
GTTTCTT C GAATTC GCGGCCGC T ACTAGT GCTTCCTCCGAAGACGTTATCAAAGAG, RFP-F
GTTTCTT C CTGCAG CGGCCGC T GCTAGC AGCACCGGTGGAGTGACGAC, RFP-R